Many methods of Folic Acid determination based on HPLC-UV (High Performance Liquid Chromotography-Ultraviolet) or HPLC-FLD (High Performance Liquid Chromatography with Postcolumn Fluorescence Derivatization) detection show low selectivity if problematic matrices are applied. Range: 0.03 - 5μg. Sensitivity: 0.03 μg. ? This method of determination of Folic Acid combines the high selectivity of immunoaffinity columns (compared to other separation materials (e.g. SAX)) with its potential to concentrate elute through use of purification by a HPLC column.