This method of content determination of fumonisins combines the high selectivity of an immunoaffinity column (IAC) with its potential to concentrate elute and additional step of purification of derivatized fumonsins by HPLC column. As said before, this instruction focuses on the handling with the IAC column. For the commodity extraction step a literature method is given. Please see below. The given apparatus (e.g. HPLC system) might serve as example among other possibilities. For your convenience, an example HPLC method for the analysis of fumonisins is given below. Range: 0.2 - 400ng FUM. Sensitivity: 0.2 - 400ng FUM. ? This instruction of fumonisins (FB1 / FB2) determination in food and feed focuses on the enrichment step of extract using immunoaffinity column (IAC) and quantification with HPLC. Accepted laboratory extraction methods could be maintained. Full performance of the IAC column is given if pronounced criteria regarding organic solvent tolerance, elution process of analyte and working range of column are followed. Many pretreatment methods of fumonisins determination in food and feed, most of them which are based on solid phase extraction (SPE) with adsorbents or strong anion resins (SAX), show low sensitivity because of interfering substances if problematic matrices are applied.