In this HGH ELISA Assay Kit, the calibrators and the patient specimens and/or controls (containing the native HGH Antigens) are first added to streptavidin coated wells. Biotinylated monoclonal and enzyme labelled antibodies are then added: these antibodies have high affinity and specificity and are directed against distinct and different epitopes of HGH. Sample Types: serum, plasma. Range: 2 - 150 μIU/ml. Sensitivity: 0.105 μIU/ml. Time: 1.5 hours ? Growth hormone is a polypeptide hormone synthesised and secreted by the anterior pituitary gland which stimulates growth and cell reproduction in humans and other vertebrate animals. GH is released from the pituitary into the bloodstream in a pulsatile manner under the regulatory control of hypothalamic somatostatin (SS) and GH-releasing factor (GHRF). Stimulators of GH secretion include, exercise, hypoglycemia, dietary protein, and estradiol. Inhibitors of GH secretion include dietary carbhoydrate and glucocorticoids.