The CD62E Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for CD62E has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD62E concentrations and unknowns are pipetted into these wells. During the first incubation, the CD62E Antigens, standards and zeros are bound this is followed by a separate incubation where the biotinylated monoclonal antibody specific for CD62E is added. Sample Types: serum, plasma, cell culture supernatant. Range: 1-32 ng/ml. Sensitivity: 0.5 ng/ml. Time: 2h10 ? The Eagle Biosciences Human CD62E ELISA Assay Kit is to be used for the in-vitro quantitative determination of soluble Endothelial Leukocyte Adhesion Molecule ELAM-1, (CD62E) in human serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant human CD62E. The Eagle Biosciences Human CD62E ELISA Assay kit is for Research Use Only.