The gp130 Kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for gp130 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known gp130 concentrations, control specimens and unknowns are pipetted into these wells. During the first incubation, the gp130 Antigens and a biotinylated monoclonal antibody specific for gp130 are simultaneously incubated. Sample Types: serum, plasma, cell culture supernatant. Range: 56.25-1800 pg/ml. Sensitivity: 15 pg/ml. Time: 1h40 ? The Eagle Biosciences Human gp130 ELISA is to be used for the in-vitro quantitative determination of soluble gp130 protein (gp130) in human serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant human gp130. The Eagle Biosciences Human gp130 ELISA Assay kit is for Research Use Only.