The CD31 Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for CD31 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD31 concentrations and unknowns are pipetted into these wells. During the first incubation, the CD31 Antigens and a biotinylated monoclonal antibody specific for CD31 are simultaneously incubated. Sample Types: serum, plasma, cell culture supernatant. Range: 0.31-10 ng/ml. Sensitivity: 0.17 ng/ml. Time: 2h45 ? The Eagle Biosciences Human CD31(PECAM-1) ELISA is to be used for the in-vitro quantitative determination of CD31/PECAM-1 (CD31) in human serum, plasma, buffered solutions or cell culture medium. The assay recognizes natural CD31. The Eagle Biosciences Human CD31 ELISA Assay kit is for Research Use Only.