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Description
This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose, then affinity purified using phospho-PDK1 (Tyr-9) peptide. The purified antibody detects a 65 kDa protein corresponding to the apparent molecular mass of PDK1 on SDS-PAGE immunoblots of human A431 cells treated with EGF, IGF-1, or pervanadate, but not in untreated cells. PDK1 (Tyr-9) reactivity was also observed in adult mouse brain.
Immunogen
Phospho-PDK1 (Tyr-9) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding tyrosine 9 in human PDK1. This sequence is highly conserved in rat and mouse PDK1.