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Description
This antibody was cross-adsorbed to unphosphorylated Profilin (Ser-138) then affinity purified using Profilin (Ser-138) peptide (without carrier). The antibody detects a 15 kDa band corresponding to the apparent molecular mass of Profilin-1 in human Jurkat and A431 cells, as well as rat PC12 cells. This reactivity is decreased by lambda phosphatase treatment, and is blocked by phospho-profilin (Ser-138) blocking peptide.
Immunogen
Phospho-Profilin (Ser-138) synthetic peptide (coupled to carrier protein) that includes amino acids surrounding Ser-138 in human Profilin-1. This sequence is well conserved in rat and mouse Profilin-1. The serine site has some homology to the conserved site (Ser-138) in Profilin-2a and Profilin-2b, but the site is not conserved in Profiin-3 and Profilin-4.