Perforin 1 (PRF1) BioAssay? ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PRF1 in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Detection Range: 0.312-20ng/ml Sensitivity: 0.114ng/ml Intra-Assay CV: <10% Inter-Assay CV: <12% Kit Components: *027348A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use *027348B: Standard, 2x1 vial 027348C: Standard Diluent, 1x20ml *027348D: Detection Reagent A (green), 1x120ul *027348E: Detection Reagent B (red), 1x120ul 027348F: Assay Diluent A, 1x12ml 027348G: Assay Diluent B, 1x12ml 027348H: TMB Substrate, 1x9ml *027348J: Positive Control, 8.18ng/ml, 1x1vial 027348K: Stop Solution, 1x6ml 027348L: Wash Buffer, 30X, 1x20ml Precaution: The Stop Solution (027348K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.