Cross-Linking and Immunoprecipitation (CLIP) is an antibody-based technique used to study RNA-protein interactions related to RNA immunoprecipitation (RIP), but differs from RIP in the use of UV radiation to cross-link RNA and the binding proteins. Unlike DNA-protein crosslinking which is done with formaldehyde, CLIP uses ultraviolet (UV) light and the crosslinking is irreversible. UV crosslinks are also more specific and only link proteins to RNAs that are in very close proximity. Further, UV crosslinks do not form between two proteins. For these reasons, UV has become the standard in RNA research. The RNA-RBP complexes are then immunoprecipitated. Due to the irreversibility of the crosslinks, the next step is digestion with a proteinase.More information, please visit the website: http://www.creative-diagnostics.com/Cross-Linking-and-Immunoprecipitation-CLIP.html