MW: 49.67 kDa. This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Legumain(LGMN) (NM_005606), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix and 1mM Na3VO4).