The FluoroSpot assay is an inventive development of the ELISpot assay and uses fluorescent detection instead of colorimetric. This allows for simultaneous detection of several analytes. The human IgG/IgM B-cell FluoroSpot kit enables detection of IgG- and IgM-secreting cells in the same well. Cross-react with IgG and IgM from non-human primates.