This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Acetylcarnitine (ALCAR) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Acetylcarnitine (ALCAR) and unlabeled Acetylcarnitine (ALCAR) (Standards or samples) with the pre-coated antibody specific to Acetylcarnitine (ALCAR). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HR