1-298aa; Full Length. MW: 50.6kD. Purity >90% as determined by SDS-PAGE. This methylase recognizes the double-stranded sequence GATATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the EcoRV endonuclease.
Characterization of the genes coding for the Eco RV restriction and modification system of Escherichia coli.Bougueleret L., Schwarzstein M., Tsugita A., Zabeau M.Nucleic Acids Res. 12:3659-3676(1984) The EcoRV restriction-modification system genes, enzymes, synthetic substrates.Kraev A.S., Kravets A.N., Chernov B.K., Skryabin K.G., Baev A.A.Mol. Biol. (Mosk.)19:236-242(1985)