Vascular endothelial growth factor (VEGF) is a signaling messenger involved in endothelial cell proliferation and angiogenesis. BioVision's rat VEGF ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A mouse monoclonal antibody specific for rat VEGF is coated on a 96-well plate. Standards and test samples are added to the wells and VEGF present in a sample is bound by the immobilized antibody. A biotinylated polyclonal antibody from goat specific for VEGF is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP sub
Size
100 assays
Concentration
n/a
Applications
This ELISA kit is used for quantitative protein detection, establishing normal range, validation of antibody array results