Competitive enzyme immunoassay for the determination of human zonulin/haptoglobin in stool.This assay is based on the method of competitive ELISA. As a first preparation step, a biotinylated zonulin tracer is added to the samples, standards and controls. Afterwards, aliquots of the treated samples, standards and controls are transferred and incubated in microtiter plate wells coated with polyclonal anti-zonulin antibodies. During the incubation, the free target antigen in the samples competes wi