This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with LN. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for LN. The competitive inhibition reaction is launched between with pre-coated LN and LN in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of LN in th