Purity ~90% (SDS-PAGE). Ppif also known as Peptidyl-prolyl cis-trans isomerase F, mitochondrial. Ppif accelerates the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and involved in regulation of the mitochondrial permeability transition pore (mPTP). It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probability of the mPTP leading to apoptosis or necrosis the requirement of the PPIase activity for this function is debated. Source: Recombinant protein corresponding to aa24-188 from rat Ppif, fused to His-Tag, expressed in E. coli. Molecular Weight: ~21.2kD (200aa), confirmed by MALDI-TOF Endotoxin: 1300nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmol of suc-AAFP-PNA/minute at 37°C in Tris-HCl pH 8.0 using chymotrypsin. AA