Human IgM (myeloma) Fc5μ fragment has been prepared from Human IgM myeloma protein by digestion with trypsin followed by column chromatography. Purity was assessed by SDS-PAGE and HPLC to be greater than 95%. A single precipitin arc was observed against anti-human IgM Fc5μ and anti-human serum when assayed by immuno-electrophoresis at a concentration of 20 mg/ml. No reaction was observed against anti-Trypsin, anti-human IgG F(ab')2, anti-human IgG F(c), anti-human Kappa or anti-human Lambda.
Size
1 mg
Concentration
1.0 mg/mL
Applications
ELISA, Immunohistochemistry, Western Blot. Human IgM (myeloma) Fc5μ fragment can be used in Western Blotting and ELISA experiments as a control reagent.