The Quantikine Human Pro-IL-1 beta is a sandwich enzyme-linked immunoassay. The capture antibody coated to the microplate is specific for the pro-piece of the molecule (first 116 residues), and the detection antibody is specific for mature IL-1 beta. Thus, the assay does not detect either the pro-piece alone or the mature form alone; it is specific instead for the intact pro-IL-1 beta. Range: 23.40 - 1500 pg/mL (Cell Culture Supernates), 31.20 - 2000 pg/mL (Serum, Heparin Plasma, EDTA Plasma). Sensitivity: 7.2 pg/mL (Cell Culture Supernates), 8.9 pg/mL (Serum, Heparin Plasma, EDTA Plasma). Time: 3 hours