MEK1 pS218-pS222 is a recombinant protein containing a polyhistidine tag expressed in Sf9. This protein is expressed in the presense of constitutively active B-raf (V600E) leading to high levels of phosphorylation of MEK1 activation residues at position S218 and S222. Analysis by SDS-PAGE resulted in a pattern consistent with purified MEK1 and was estimated to be greater than 90% pure.
Size
10 μg
Concentration
n/a
Applications
Western Blot. MEK1 pS218-pS222 is an activated recombinant protein suitable as a control for polyclonal or monoclonal anti-MEK1 in immunological assays. Activated MEK1 can be used in vitro to phosphorylate ERK protein. For western blot use at 50 ng or less. For other assays concentration is user optimized.
Other Names
MAP2K, MEK, MEK1, MKK1, PRKMK1, MEK-1 recombinant protein