ELISA, Western Blot. This protein-A purified antibody has been tested for use in Western blotting. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 33.9 kDa in size corresponding to Borrelia burgdorferi Flagellin protein by Western blotting in the appropriate cell lysate or extract.
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Description
This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Lyme Borrelia spp. Flagellin protein. A BLAST analysis was used to suggest cross-reactivity with Flagellin from B. burgdorferi, garinii, and valaisiana sources based on 100% homology with the immunizing sequence.. Cross-reactivity with Flagellin from other sources has not been determined.
Immunogen
MBP-fusion protein corresponding to Borrelia burgdorferi Flagellin protein.
Casselli, T., Tourand, Y., & Bankhead, T. (2012). Altered murine tissue colonization by Borrelia burgdorferi following targeted deletion of linear plasmid 17-carried genes. Infection and immunity, 80(5), 1773-1782.Rogovskyy, A. S., & Bankhead, T. (2013). Variable VlsE is critical for host reinfection by the Lyme disease spirochete. PloS one, 8(4), e61226.