ELISA, Immunohistochemistry, Western Blot. This secondary peroxidase conjugated antibody anti-Mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays. Mouse IgM (H&L) peroxidase conjugated antibody has been assayed against 1.0 ug of mouse IgM in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:40,000 of the reconstitution concentration is suggested for this product.
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Description
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Rabbit Serum. This reagent primarily recognizes Mouse IgM however some light chain cross reactivity will occur against other mouse immunoglobulins.
Immunogen
Anti-Mouse IgM (H&L) was produced by repeated immunization with mouse IgM whole molecule in rabbit.