FLISA, Flow Cytometry, Immunohistochemistry, IF Microscopy, Western Blot. Fluorescent Rabbit TrueBlot? Antibody DyLight? 800 is provided as a lyophilized powder. In order to conserve reagent, we recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mls). If used conservatively at 2.5mls/blot, cat. 18-4516-32 (100μL) will yield enough reagent for 200 blots.Note that there are three key procedural considerations:1. Protein A or G should not be used for the immunoprecipitation. For immunoprecipitation, anti-rat IgG beads, or anti-rabbit IgG beads should be used for rat or rabbit immunoprecipitating antibodies, respectively.2. Immunoprecipitate should be completely reduced.3. Bovine Serum Albumin, at low concentrations, should be used as the blocking protein for the immunoblot. DO NOT USE MILK.Fluorescence technology is widely used to detect proteins. However, many common visible fluorophores often result in considerable background fluorescence in the visible range. Visible fluorophores are rarely used for membrane-based protein detection because of this high background. DyLight? 800 and DyLight? 680 antibody and reagent conjugates are specifically designed for protein detection methods that use longer-wavelength, near-infrared (IR) fluorophores to visualize proteins in western blotting and other applications. Very low background fluorescence in the IR range provides for a much higher signal-to-noise ratio than visible fluorophores. Detection levels in the picogram range on Western blots rival the sensitivity of chemiluminescence on film. DyLight? 800 conjugates are optimized for the Odyssey? Infrared Imaging System developed by LI-COR. DyLight? 800 conjugates are also suitable for immunofluorescence microscopy using commercially available excitation/emission filters in the 780nm/820nm range. Dual simultaneous labeling in western blots or microscopy is achieved when DyLight? 800 conjugates are used in conjunction with DyLight? 680 conjugates. DyLight? 800 and DyLight? 680 conjugates provide an ultra-sensitive and convenient alternative to standard chemiluminescent protein detection methods, as well as a valuable tool for multicolor imaging.
Reviews / Ratings
If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY.
Description
Fluorescent Rabbit TrueBlot? Antibody DyLight? 800 Conjugate was prepared from tissue culture supernatant by Protein G affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Reactivity is observed against native Rabbit IgG by both Western blot and ELISA.
Immunogen
n/a
Other Names
DL800, TrueBlot, DL800 TrueBlot ULTRA, DyLight? 800 TrueBlot, TrueBlot for IP/WB, TrueBlot for immunoprecipitation, TrueBlot for western blotting, Fluorescent TrueBlot, Rb TrueBlot, Infrared, IR, NIR, IR800