FLISA, Flow Cytometry, Immunohistochemistry, IF Microscopy, Western Blot. Fluorescent Rabbit TrueBlot? Antibody DyLight? 680 is provided as a lyophilized powder. In order to conserve reagent, we recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mls). If used conservatively at 2.5mls/blot, cat. 18-4416-32 (100μL) will yield enough reagent for 40 blots.Note that there are three key procedural considerations:1. Protein A or G should not be used for the immunoprecipitation. For immunoprecipitation, anti-rat IgG beads, or anti-rabbit IgG beads should be used for rat or rabbit immunoprecipitating antibodies, respectively.2. Immunoprecipitate should be completely reduced.3. Milk should be used as the blocking protein for the immunoblot.
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Description
Fluorescent Rabbit TrueBlot? Antibody DyLight? 680 Conjugate was prepared from tissue culture supernatant by Protein G affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Reactivity is observed against native Rabbit IgG by both Western blot and ELISA.
Immunogen
n/a
Other Names
DL680, TrueBlot, DL680 TrueBlot ULTRA, DyLight? 680 TrueBlot, TrueBlot for IP/WB, TrueBlot for immunoprecipitation, TrueBlot for western blotting, Fluorescent TrueBlot, Rb TrueBlot