ELISA, Immunohistochemistry, Western Blot. SKG-1 Antibody antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~49 kDa in size corresponding to SGK-1 by western blotting in the appropriate cell lysate or extract.
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Description
Anti-SKG-1 Antibody is directed against human SGK-1 protein. The product was protein A purified from monospecific antiserum. A BLAST analysis was used to suggest reactivity with this protein from human, macaque and chimpanzee sources based on 100% homology for the immunogen sequence. This antibody is expected to cross react with SGK-1 homologues from mouse, rat, dog, chicken, bovine, rabbit and spiny dogfish based on an 92% sequence homology for the immunogen (12/13) from these sources. Cross reactivity with SGK-1 homologues from other sources has not been determined.
Immunogen
SKG-1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 419-431 of human SGK -1.