ELISA, Flow Cytometry, Immunohistochemistry, Western Blot. This Anti-ATM Protein Kinase pS1981 (MOUSE) Monoclonal Antibody clone has been optimized for IHC, but may also be used for western blotting, flow cytometry, immunoprecipitation, or immunofluorescence microscopy. Indirect immunoperoxidase staining on formaldehyde-fixed, de-paraffinized tissue sections and/or formalin-fixed cultured cells are recommended when using this antibody as described in Bartkova et al 2005. Product p/n 200-301-400 produced from clone 10H11.E12 has been optimized for WB, IP and IF.
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Description
This Protein A Purified Mab antibody is directed against human ATM and is useful in determining its presence by immunohistochemistry. This monoclonal anti-ATM antibody recognizes the phosphorylated form of the protein in native and over-expressed proteins found in various tissues and extracts. Reactivity is observed against human and mouse ATM. Cross reactivity with ATM from other mammalian sources has not been tested.
Immunogen
This antibody was produced from a synthetic peptide S-L-A-F-E-E-G-Sp-Q-S-T-T-I-S-S corresponding to aa 1974-1988 of human ATM.
Bakkenist, C. J. & Kastan, M. B. (2003). DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation. Nature 421, 499-506.Kitagawa R, Bakkenist CJ, McKinnon PJ, Kastan MB. (2004) Phosphorylation of SMC1 is a critical downstream event in the ATM-NBS1-BRCA1 pathway. Genes Dev. 18(12):1423-38.Bartkova J, Bakkenist CJ, Rajpert-De Meyts E, Skakkebaek NE, Sehested M, Lukas J, Kastan MB, Bartek J. (2005) ATM Activation in Normal Human Tissues and Testicular Cancer. Cell Cycle 4;(6) [Epub ahead of print].