Titrated value sufficient to run approximately 10 mini blots.
Applications
Western Blot. Anti-GAP43 (Rabbit) antibody is suitable for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 50 kDa in size corresponding to neuromodulin protein phosphorylated at Ser41 in the appropriate cell lysate or extract. In some tissues, the antibody also recognized a higher molecular weight protein that may be an aggregate or oligomer.
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Description
Anti-GAP43 pS41 antibody was affinity purified from monospecific antiserum by immunoaffinity purification. This antibody is specific for the phosphorylated forms of the protein. Immunolabeling is completely eliminated by treatment with lambda-Ptase. Cross reactivity with GAP43 from other species has not been determined, however, the sequence of the immunogen shows 100% homolgy to bovine, canine, chicken, finch, human, mouse, non-human primates, Xenopus and zebra fish.
Immunogen
Anti-Neuromodulin Antibody was produced by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser41 of GAP43.
Other Names
Neuromodulin, Axonal membrane protein GAP-43, Growth-associated protein 43, Protein F1, Neural phosphoprotein B-50, pp46