ELISA, Western Blot. Each kit contains one unit of the following products: Anti-NFKB p50 (NFKB1) (RABBIT) Antibody (100 μL) Anti-NFKB p65 (Rel A) (RABBIT) Antibody (100 μL) Anti-NFKB cRel (RABBIT) Antibody (100 μL) Anti-IKBα C-terminal (RABBIT) Antibody (100 μL) Anti-RABBIT IgG (H&L) (GOAT) Peroxidase Conjugated Antibody (100 μg) CONTROL PEPTIDE for Anti-NFKB p50 (NFKB1) (RABBIT) Antibody (50 μg)CONTROL PEPTIDE for Anti-NFKB p65 (Rel A) (RABBIT) Antibody (50 μg) CONTROL PEPTIDE for Anti-NFKB cRel (RABBIT) Antibody (50 μg) CONTROL PEPTIDE for Anti-IKBα C-terminal (RABBIT) Antibody ( (50 μg)
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Description
NFKB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFkB bound to IkB. NFkB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFkB subunit p65, similar to p50/p65 heterodimers. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IkB-a. IkB-a binds to the p65 subunit, preventing nuclear localization and DNA binding. Low levels of p52 and p50 homodimers can also exist in cells.IKB-alpha inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, IKBA becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. Anti-NFkB and Anti-IKBa Antibodies are ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction and Immunology research.