ELISA, Gel Shift, Western Blot. This product was assayed by immunoblot and found to be reactive against NFkB cRel at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302. Anti-NFkB cRel is suitable for the detection by immunoblot of human NFkB cRel. No reaction was observed against the anologous Mouse protein. This product was also tested in a gel supershift assay and found to be reactive against all cRel containing human NFkB complexes using 0.5 to 1.0 μl per assay. This product was assayed by immunoblot and found to be reactive against NFkB cRel at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302. Anti-NFkB cRel is suitable for the detection by immunoblot of human NFkB cRel. No reaction was observed against the anologous Mouse protein. This product was also tested in a gel supershift assay and found to be reactive against all cRel containing human NFkB complexes using 0.5 to 1.0 μl per assay. This product was assayed by immunoblot and found to be reactive against NFkB cRel at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302. Anti-NFkB cRel is suitable for the detection by immunoblot of human NFkB cRel. No reaction was observed against the anologous Mouse protein. This product was also tested in a gel supershift assay and found to be reactive against all cRel containing human NFkB complexes using 0.5 to 1.0 μl per assay.
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Description
This product was prepared from monospecific antiserum by delipidation and defibrination. Anti-NFkB cRel may react non-specifically with other proteins. Control peptide (code #100-4166p) will compete only with the specific reaction of antiserum with the NFkB cRel subunit.
Immunogen
NFkB cRel peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).