ELISA, Immunohistochemistry, Western Blot. This phospho-specific polyclonal antibody was tested by immunoblotting and ELISA. By ELISA, the antibody was found to be reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide. Immunoblotting will detect human and mouse NF2 (Merlin). Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.
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Description
This affinity purified antibody is directed against human NF2 (Neurofibromatosis 2 gene product). The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. This phospho-specific polyclonal antibody is specific for phosphorylated pS518 of human NF2 (neurofibromatosis 2 gene product). Reactivity with non-phosphorylated Human NF2 (Merlin) is minimal by ELISA. Cross-reactivity with NF2 (Merlin) occurs in mouse tissue. Reactivity with NF2 from other sources has not been determined.
Immunogen
Human NF2 (Merlin) phospho-peptide corresponding to a region of the human protein surrounding S518 and conjugated to Keyhole Limpet Hemocyanin (KLH).
Lallemand D, Saint-Amaux AL, Giovannini M. (2009) Tumor-suppression functions of merlin are independent of its role as an organizer of the actin cytoskeleton in Schwann cells. J Cell Sci. 2009 Nov 15;122(Pt 22):4141-9. doi: 10.1242/jcs.045914.