ELISA, Western Blot. Anti-Alkaline Phosphatase (E. Coli) Antibody has been assayed against 1.0 ug of Alkaline Phosphatase [E.coli] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for Anti-Alkaline Phosphatase (E. Coli) Antibody.
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Description
Anti-Alkaline Phosphatase (E. Coli) Antibody was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Alkaline Phosphatase [E.coli]. No cross reactivity occurs against the mammalian form of Alkaline Phosphatase.
Epstein, Kiechle, Artiss, and Zak. The Clinical Use of Alkaline Phosphatase Enzymes. Clin Lab Med. (1986): Sep. 6 (3):491-505.Lange, Millan, and Stigbrand, et. al. Placental Alkaline Phosphatase as a Tumor Marker for Seminoma. Cancer Res. (1982): Aug. 42 (8):3244-7.