Recombinant full length human HSP90β was expressed by baculovirus in Sf9 insect cells using an N-Terminal his epitope. The purity was determined to be >90% by densitometry.
Size
20 μg
Concentration
0.2 μg/μL
Applications
Western Blot. HSP90β Protein is stored in 50mM NaPhosphate pH7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.2mM DTT, 25% glycerol. HSP90β Protein is suitable for use in Western Blot and ATPase Assay. Expect a band approximately ~91kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.