This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Gonadotropin Releasing Hormone (GnRH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Gonadotropin Releasing Hormone (GnRH) and unlabeled Gonadotropin Releasing Hormone (GnRH) (Standards or samples) with the pre-coated antibody specific to Gonadotropin Releasing Hormone (GnRH). After incubation the unbound conjugate is washed of