Chemiluminescent FemtoMax? Super Sensitive HRP Substrate
Description
FemtoMax? Super Sensitive Chemiluminescent HRP Substrate is an extremely sensitive, nonradioactive, enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP). FemtoMax? is designed for both western blotting and enzyme immunoassay (EIA) use. FemtoMax? easily allows for the detection of femtogram (10-15) amounts of antigen using photographic film or other imaging methods, including highly sensitive CCD cameras. Blots can be repeatedly exposed to X-ray film to obtain optimal results or stripped of detection reagents and re-probed. Use the same blotting conditions for FemtoMax? as you would when using Amersham ECL Plus? Substrate or Pierce SuperSignal? West Femto Substrate.
Size
110 mL
Concentration
n/a
Applications
ELISA, Western Blot. Prepare FemtoMax? Super Sensitive Chemiluminescent HRP Substrate for use in microwell or membrane applications by mixing 1 mL of Luminol Reagent (Reagent A) with 1 mL of Reaction Buffer (Reagent B). Mix well. Protect from light. Larger or smaller volumes of the substrate can be prepared by mixing components at the same 1:1 ratio. FemtoMax? Super Sensitive Chemiluminescent HRP Substrate is a highly sensitive detection reagent. Always carefully optimize all components of individual assays (antigens, antibodies, conjugates...) to minimize background reactivity associated with non-specific binding.
Other Names
Peroxidase Substrate, ECL HRP Substrate, Chemiluminescent Femtomax? Super Sensitive horseradish peroxidase (HRP) Substrate For Microwell And/Or Membrane (2 Component System)