Mouse IgM was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum and anti-Mouse IgM (μ chain specific). No reaction was observed against anti-Mouse IgG F(c). Some light chain cross-reactivity will occur with anti-Mouse IgG.
Size
1 mg
Concentration
1.0 mg/ml
Applications
ELISA, Western Blot. Mouse IgM whole molecule can be utilized as a control or standard reagent in SDS, Western Blotting, and ELISA experiments.