This product was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Mouse Serum and anti-Mouse IgM (μ chain specific). No reaction was observed against anti-Mouse IgG F(c). Some light chain cross reactivity will occur with anti-Mouse IgG.
Size
500 μg
Concentration
n/a
Applications
Western Blot. Mouse IgM whole molecule Fluorescein conjugation is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.