WM852 is a metastaic melanoma cell line with epithelial morphology. This cell line was established from the metastatic site (abdomen) in a 63-year -old male. This cell line contains a Q61R mutation at position 61 in the N-RAS gene. The Q61R is the most common NRAS mutation found in melanoma that is thought to occur due to UV and radiation exposure. This mutation leads to production of a constitutively active N-RAS protein that directs cells to grow and divide constantly. This cell line also expresses PTEN loss of function including hemizygous deletion and is wild type for BRAF, c-KIT and CDK4. WM852 cells produce xenograft tumors when injected into immunocompromised mice.
Size
1 mL
Concentration
10.0 million cells/ml
Applications
The key applications of these cell lines include genetic studies, xenograft production, drug testing, and drug target discovery. These cell line models can be used in various biological assays, and for identifying critical target genes, and cell signaling pathways.
Other Names
Human Melanoma, patient derived tumor, tumor models, skin cancer, xenograft
Friedegund Meier et al. Human Melanoma Progression in Skin Reconstructs: Biological Significance of bFGF. Am J Pathol. (2000); 156(1): 193-200. doi: 10.1016/S0002-9440(10)64719-2