BlockOut? (2X) - Universal Blocking Buffer for Western Blotting
Description
BlockOut? Blocking buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in TRIS buffered saline at pH 7.6 with thimerisol added as an antimicrobial agent.
Size
50 mL
Concentration
n/a
Applications
Western Blot. BlockOut? is a 2X concentrated stock solution. Prepare a 1X working solution by diluting 1 part 2X concentrate with 1 parts TBS or equivalent. This product allows for superior signal detection and lower background noise in colorimetric, chemiluminescent, and fluorescent western blotting and other applications making it a convenient general purpose blocking agent. Antibody conjugates prepared with IRDye? 800 and IRDye? 700DX (Licor), Cy2?, Cy3?, Cy3.5?, Cy5? and Cy5.5? (GE Healthcare), DyLight?405, DyLight? 549, DyLight? 649, DyLight? 680, and DyLight? 800 (Thermo Fisher/Pierce) and Alexa Fluor? 488, Alexa Fluor? 532, Alexa Fluor? 546, Alexa Fluor? 647 and Alexa Fluor? 680 (Invitrogen/Molecular Probes) and ATTO (Atto-Tec) have been validated on various platforms using this product with superior results compared to other commercially available products. In the infrared range, where little to no autofluorescence occurs, specific signal is sharply evident from any background giving the best possible signal-to-noise ratio. This allows for detection levels in the picogram range which rivals the sensitivity of chemiluminescence on film for western blotting. Superior results are also seen when this product is used for simultaneous labeling (multiplex) in western blots or microscopy using various fluorochrome combinations for multicolor imaging. Membranes blocked with BlockOut? can be dried and are very stable.
Other Names
multiplex Blocking Buffer, Immunoblot Blocking Buffer, Blocking Solution, Blocking Buffer Western Blot, Western Blot Blocking Buffer, Alexa Dye Blocking Buffer, DyLight Blocking Buffer, colorimetric, chemiluminescent blocking buffer, WB block, BlockOut, Block out
Gordon, R; Hogan, CE; Neal, ML; Anantharam, V; Kanthasamy, AG; Kanthasamy , A. A simple magnetic separation method for high-yield isolation of pure primary microglia. Journal of Neuroscience Methods 194 (2011) 287-296