Recombinant Escherichia coli 30S ribosomal protein S19 (rpsS)
Description
Purity > 90% as determined by SDS-PAGE. MW: 14.3kDa. Full Length of Mature Protein: 2-92aa. N-terminal 6xHis-tagged. Escherichia coli (strain K12). In the E.coli 70S ribosome in the initiation state it has been modeled to contact the 23S rRNA of the 50S subunit forming part of bridge B1a; this bridge is broken in the model with bound EF-G. The 23S rRNA contact site in bridge B1a is modeled to differ in different ribosomal states, contacting alternately S13 or S19. In the 3.5 angstroms resolved ribosome structures the contacts between L5, S13 and S19 bridge B1b are different, confirming the dynamic nature of this interaction. Bridge B1a is not visible in the crystallized ribosomes due to 23S rRNA disorder.