The Eagle Biosciences 24-sample kit's PCR mix a piece of DNA sequence around codon 3500 can be amplified and lateron incubated at elevated temperature with a bacterial restriction enzyme, which can recognize and cut the amplimere, when an A-mutation is present and not in the case of a normal G. The effect of the restriction experiment can be controlled by comparing the length of uncut and cut fragments to DNA of known size with a fluorescently coloured electrophoresis gel and UV light. Copyright: the intellectual property for this method owns to Dr.M.Eβrich, Denzlingen/Freiburg (Germany). Sample Types: DNA. ? The Eagle Biosciences MutaGEL? ApoB100 (Codon 3500) is a RFLP-coupled PCR test kit for the inspection of codon 3500 of the human apolipoprotein-B gene, with the purpose to detect DNA base G (normal) or A (mutant), causative for tanslated arginine or glutamine at this position in the ApoB100 protein. The Eagle Biosciences MutaGEL? ApoB100 is for research use only and is not intended for diagnostic or therapeutic procedures.