ELISA, Immunohistochemistry, Western Blot. Anti-Notch-1 has been tested in IHC and ELISA. This antibody is useful in western blot. Specific conditions for reactivity should be optimized by the end user.
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Description
Notch1 antibody is directed against human NOTCH 1. No reaction is detected against NOTCH 2. No reactivity was observed against Mouse Notch. Other species have not been tested.
Immunogen
Anti-Notch antibody was prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues 2488-2502 of human Notch 1. A residue of cysteine was added to the amino terminal end to facilitate coupling.
Spyros Stylianou, Clarke RB, and Brennan K (2006) Aberrant Activation of Notch Signaling in Human Breast Cancer. Cancer Res February 1, 2006 66; 1517 doi: 10.1158/0008-5472.CAN-05-3054