Collagen Type I (COL1) BioAssay? ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of COL1 in rat serum, plasma and other biological fluids. Detection Range: 0.625-40ng/ml Sensitivity: 0.224ng/ml Intra-Assay: CV<10% Inter-Assay: CV<12% Kit Components: *024261A: Microtiter Strips, 1x48 wells or1x96 wells *024261B: Standard, 2x1vial 024261C: Standard Diluent, 1x10ml or 1x20ml *024261D: Detection Reagent A (green), 1x60ul or 1x120ul *024261E: Detection Reagent B (red), 1x60ul or 1x120ul 024261F: Assay Diluent A, 1x6ml or 1x12ml 024261G: Assay Diluent B, 1x6ml or 1x12ml 024261H: TMB Substrate, 1x14.5ml or 1x9ml *024261J: Positive Control, 24.6ng/ml, 1x1vial (Lyophilized) 024261K: Stop Solution, 1x3ml or 1x6ml 024261L: Wash Buffer, 30X, 1x10ml or 1x20ml Precaution: The Stop Solution (024261K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.