Interleukin 12, Human, BioAssay? ELISpot Development Kit (Interleukin 23 p40, IL-12/IL-23 p40, CLMF2, IL12B, IL-12B, NKSF2) from UNITED STATES BIOLOGICAL
Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40kD (p40) subunit and a 35kD (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells. Kit Components: 1. Capture Antibody, 1x1vial 2. Biotinylated-conjugated Detection Antibody, 1x1vial Required but not Included: ELISpot Blue Color Module or equivalent PBS: 37mM NaCl, 2.7mM KCl, 8.1mM Na2HPO4, 1.5mM KH2PO4, pH 7.2-7.4, 0.2um filtered. Wash Buffer: 0.05% Tween 20 in PBS Blocking Buffer: 1% BSA, 5% Sucrose in PBS Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2um filtered