The Quantikine Human Pro-MMP-13 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure pro-MMP-13 in cell culture supernates. It contains NS0-expressed recombinant human pro-MMP-13 and antibodies raised against the recombinant factor. The Quantikine kit will not detect recombinant human active MMP-13 existing in either free or TIMP-bound form. Natural human pro-MMP-13 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human pro-MMP-13. Range: 78.00 - 5000 pg/mL. Sensitivity: 21.3 pg/mL. Time: 4.5 hours