Human NF-kB Family Member Antibody Sampler Kit, BioAssay?
Description
Transcription factors of the nuclear factor kB (NF-kB)/Rel family play a pivotal role in inflammatory and immune responses. There are five family members in mammals: RelA, c-Rel, RelB, NF-kB1 (p105/p50), and NF-kB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-kB is sequestered in the cytoplasm by IkB inhibitory proteins. NF-kB-activating agents can induce the phosphorylation of IkB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-kB to enter the nucleus where it regulates gene expression. NIK and IKKalpha (IKK1) regulate the phosphorylation and processing of NF-kB2 (p100) to produce p52, which translocates to the nucleus). Specificity: Human Intended Use: This BioAssay? kit contains reagents to examine total protein levels of the five NF-