The mTORC1 kinase complex is a critical regulator of cell growth. Its activity is modulated by energy levels, growth factors, and amino acids via signaling through Akt, MAPK, and AMPK pathways. Recent studies found that the four related GTPases, RagA, RagB, RagC, and RagD, interact with raptor within the mTORC1 complex. These interactions are both necessary and sufficient for mTORC1 activation in response to amino acid signals. Specificity: Human Intended Use: The Rag Protein Antibody Sampler BioAssay? Kit provides an economical means to evaluate the regulation of mTORC1 signaling. The kit contains enough primary and secondary antibodies to perform four Western blot experiments per primary antibody. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:2000 Optimal dilutions to be determined by the researcher. Kit Components: 391822, mTOR, Pab, 1x20ul 394013, RRAGA, Pab, 1x20ul 393683, Rag A/B, Pab, 1x20ul 393684, Rag