The reconstituted Veri-Cells? Activated (Cytokine) PBMC can be used for immunofluorescent staining and multi-color flow cytometry assays. The cells can be used as controls for cytokine staining with minimal variability across experiments. These cells have been verified to work with commonly tested cell surface (CD3, CD4, CD8, CD19, CD56) as well as cytokine markers such as IL-2, IL-3, IL-4, IL-5, IL-8, IL-13, IL-17, GM-CSF, IFN gamma, and TNF alpha. Some targets such as IL-5 are rare and will require the acquisition of at least 100,000 CD3 events. This product contains human cells, a potentially biohazardous material. Blood used in preparation of these samples was tested and found to be negative for antibody and nucleic acid testing against Human Immunodeficiency virus (HIV), Hepatitis B and C virus and HTLV (Human T-Lymphotrophic virus I and II), surface antigen for Hepatitis B (HBsAg) virus and syphilis and West Nile Virus using FDA approved methods. Biological tests are not 100% accurate. Use standard precautions when handling, and treat as if the product is capable of transmitting disease. When handling or disposing, follow precautions described in CDC and FDA recommendations and OSHA Bloodborne Pathogen guidelines.