The determination of albumin is carried out as direct competitive ELISA. Rat albumin has been pre-coated onto a microplate. During incubation the binding of an enzyme-linked anti-albumin(rat)-antibody to the wells is inhibited by albumin from samples/standards. After washing away any unbound antibody, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is reverse proportional to the albumin con centration. Sample Types: urine. Range: 10 - 10240 ng/ml. Sensitivity: 0.4 μg/ml. Time: 2 hours ? The Eagle Biosciences Rat Albumin ELISA Assay Kit is designed for the quantitative determination of albumin in rat urine samples. The Rat Albumin ELISA Assay Kit is for research use only and should not be used in diagnostic procedures.