RSPO-3, Recombinant, Human (R-Spondin 3, Cristin-1) (BSA & Azide Free)
Description
Purity ≥95% (SDS-PAGE). R-Spondin 3 (RSPO3, roof plate-specific spondin3), also called cysteine-rich and single thrombospondin domain containing-1 (Cristin 1), is a ~31kD secreted protein that shares ~40% amino acid identity with the other three R-Spondin family members (1, 2). All are positive modulators of Wnt /b-catenin signaling, but each has a distinct expression pattern (1-4). Like other R-spondins, R-Spondin3 contains two adjacent cysteine-rich furin-like domais (aa33-135) with one potential N-glycosylationsite (aa36) , followed by a thrombospondin (TSP-1) motif (aa147-207) and a region rich in basic residues (aa211-269). Only the furin-like domains are needed for b-catenin stabilization (2). Within aa21-209, human R-Spondin 3 shares 93%, 92%, 97%, 96% and 92% aa identity with mouse, rat, equine, bovine and canine R-Spondin 3, respectively. Potential isoforms of 279 and 297 aa diverge at aa 210 and 276, respectively (5). Mouse R-Spondin 3 is critical for development of the plac